Search results for " CLSM"
showing 3 items of 3 documents
The seed endosphere of Anadenanthera colubrina is inhabited by a complex microbiota, including Methylobacterium spp. and Staphylococcus spp. with pot…
2018
Background and aims: Plant seeds are emerging micro–habitats, whose importance as reservoir and vector of beneficial microbes just begins to be recognized. Here we aimed to characterize the bacterial microbiota of the Anadenanthera colubrina seed endosphere, with special focus to beneficial traits and to the colonization pattern. Methods: Cultivation–dependent (isolation from surface–sterilized seeds) and cultivation–independent (pyrosequencing of 16S rRNA gene from metagenomic seed DNA) analyses, functional tests and microscopical investigations (fluorescence in situ hybridization coupled with confocal laser scanning microscopy (FISH-CLSM) were performed. Results: We isolated several Methy…
Confocal microscopy study of the distribution, content and activity of mitochondria during Paracentrotus lividus development
2007
Summary In the present paper we applied confocal microscopy andfluorescence technologies for studying the distribution andthe oxidative activity of sea urchin ( Paracentrotus lividus )mitochondria during development, by in vivo incubating eggsand embryos with cell-permeant MitoTracker probes. Wecalculated, by a mathematical model, the intensity values, the variations of intensity, and the variation index of incorporatedfluorochromes. Data demonstrate that mitochondrial massdoes not change during development, whereas mitochondrialrespirationincreases.Inaddition,startingfrom16blastomeresstage, some regions of the embryo contain organelles moreactive in oxygen consumption. Introduction The con…
Prime indicazioni sulle biocenosi associate alle mummie delle Catacombe dei Cappuccini di Palermo
2009
La fama del convento, costruito dai Cappuccini di Palermo nel 1533, è dovuta in gran parte alle famose "Catacombe". L’origine delle catacombe si fa risalire al 1599 circa, quando i frati, sfruttando una preesistente cavità naturale al di sotto dell’altare maggiore della chiesa, trasferirono le salme di 40 frati precedentemente sepolti presso il lato meridionale della chiesa stessa. I frati iniziarono ad ampliare le catacombe, scavando nel sottosuolo, in relazione al crescente numero di nuove salme che nel tempo si conservavano. Allo stato attuale le catacombe sono una sorta di cimitero sotterraneo, in uso nel XVII secolo, costituito da lunghe gallerie scavate nel tufo per un’estensione di c…